Vol.71,No.4,1976 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS AGGLUTINATION OF A TRANSFORMED MOUSE CELL LINE AND A VARIANT SUBLINE WITH CONCANAVALIN A: EFFECT OF TEMPERATURE AND TIME OF LECTIN INCUBATION

Colchicine treatment enhanced Con A-mediated agglutination of erythrocytes to LM cells (LM is a "spontaneously" transformed mouse line) incubated for brief periods with Con A at 22“ C. Longer incubations with Con A at 22' C rendered colchicine treated cells less agglutinable than untreated cells. Even short incubation times with Con A at higher temperature (37' C) rendered colchicine treated LM cells less agglutinable than their untreated counterparts. Below 15" C, colchicine treated cells remained more agglutinable than untreated cells even after long periods of Con A treatment. Cells of a variant clone (Rl) isolated from LM by negative selection with concanavalin A exhibited increased substratum adhesiveness and an absolute serum requirement. LM and variant cells exhibited a differential reponse to colchicine treatment, the variant subline reguiring longer periods of colchitine treatment to elicit changes in morphology and agglutinability.